General Objective: Develop a prototype of a genetic circuit, using the model organism E.coli, in order to achieve an efficient and simple detection of the amino acids Phe and Tyr.
Specific objectives:
- Obtain synthetic sequences and specific bacterial strains for the development of the genetic circuit. Refine the designs of the genetic circuit to improve its operation and efficiency.
- Assemble the modules of the genetic circuit in the E. coli bacteria, ensuring correct integration and functionality of the system.
- Evaluate the ability to detect and accurately quantify the target amino acids in the biosensor strain by testing with amino acid standards.
- Transfer knowledge of the genetic circuit and biosensor strain to facilitate its reproduction in other laboratories.
Participating Institutions:
USFQ, U CUENCA, UTPL.
Participants:
Project Director Vanessa Isabel Romero Aguilar
- Juan Carlos Collantes Vela
- Juan Carlos Pozo Palacios
- María de Lourdes Huiracocha Tutiven
- Gabriele Davide Bigoni Ordoñez
- Natali Elizabeth Solano Cueva
- Jorge Geovanny Figueroa Hurtado
Awarded budget: $49500,00
Project status: In process
