General Objective: To evaluate the activation efficiency of cyclophosphamide by the improved P450 enzyme using an in vivo reporter system and determine its kinetic characteristics.
Specific objectives:
- Construct variants of the P450 enzyme by ancestral sequence reconstruction (ASR) and point mutations.
- Determine the levels of stability and expression in the P450 enzyme variants obtained in objective 1.
- Quantify the activity of P450 enzyme variants on cyclophosphamide (CPA) using an in vivo reporter system.
- To evaluate the kinetic properties of the most active P450 enzyme variants obtained from objective 2.
Participating Institutions:
UTE, UCUENCA, USFQ.
Participants:
Project Director Saskya Estefany Carrera Pacheco
- Johana Janina Zúñiga Miranda
- Manuel Alfredo Campoverde Cisneros
- Enrique Eduardo Terán Torres
Awarded budget: $35222,00
Project Status: Completed
