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Development of a phenylalanine and tyrosine detection system using prokaryotic genetic circuits

General Objective: Develop a prototype of a genetic circuit, using the model organism E.coli, in order to achieve an efficient and simple detection of the amino acids Phe and Tyr.

Specific objectives:

  • Obtain synthetic sequences and specific bacterial strains for the development of the genetic circuit. Refine the designs of the genetic circuit to improve its operation and efficiency.
  • Assemble the modules of the genetic circuit in the E. coli bacteria, ensuring correct integration and functionality of the system.
  • Evaluate the ability to detect and accurately quantify the target amino acids in the biosensor strain by testing with amino acid standards.
  • Transfer knowledge of the genetic circuit and biosensor strain to facilitate its reproduction in other laboratories.

Participating Institutions:

USFQ, U CUENCA, UTPL.

Participants:

Project Director Vanessa Isabel Romero Aguilar

  • Juan Carlos Collantes Vela
  • Juan Carlos Pozo Palacios
  • María de Lourdes Huiracocha Tutiven
  • Gabriele Davide Bigoni Ordoñez
  • Natali Elizabeth Solano Cueva
  • Jorge Geovanny Figueroa Hurtado

Awarded budget: $49500,00

Project status: In process