General Objective: To evaluate the leishmanicidal, cytotoxic and genotoxic activities of bis(spiro-2,4-dihydro-3H-pyrazol-3-one) cyclopropanes transported in nanocarriers.
Specific objectives:
- Synthesize bis(spiro-2,4-dihydro-3H-pyrazol-3-one) cyclopropanes to be incorporated into the nanocarriers.
- Synthesize nanoparticles (lipid, mesoporous silica and metal organic frameworks) to be used as vehicles for the synthesized spirocyclopropanes.
- Encapsulate the spirocyclopropane molecules in the three types of synthesized nanoparticles.
- To evaluate the in vitro cytotoxic activity of bis(spiro-2,4-dihydro-3H-pyrazol-3-one) cyclopropanes carried in nanocarriers against Leishmania mexicana, (intracellular promastigotes and amastigotes), macrophages and hepatocytes.
- Determine the relationship between the leshmanicidal activity of bis(spiro-2,4-dihydro-3H-pyrazol-3-one) cyclopropanes and apoptosis as a cell death process induced in Leishmania mexicana.
- To evaluate the in vitro genotoxicity of bis(spiro-2,4-dihydro-3H-pyrazol-3-one) cyclopropanes carried in nanocarriers against Leishmania mexicana, macrophages and hepatocytes.
Participating Institutions:
UTE, UCE, USFQ, UTPL, UV.
Participants:
Project Director Jorge Humberto Heredia Moya
- Diego Roberto Flores Oña
- Patricio – Rojas Silva
- Eduardo David Valencia Gonzaga
- Erika Belen Muñoz Salazar
- Ronny Javier Pibaque Sánchez
- Juan Carlos Romero Benavides
- Natalia Catalina Bailon Moscoso
- Ana Paulina Arévalo Jaramillo
- Luis Miguel Guaman Ortia
- Jaqueline Elizabeth Guaman Hurtado
- Raquel Eugenia Galian Baca
- Julia Perez Prieto
- Emilio José Pardo Marín
Awarded budget: $50000,00
Project Status: Completed
