General Objective: Study the mechanism of action of compounds derived from marine bacteria, capable of inhibiting the growth and pathogenicity of V. parahaemolyticus, in order to evaluate their potential use in products for the control of infections of this aquaculture pathogen in farming systems. crop.
Specific objectives:
- To evaluate the inhibitory activity of probiotic vibrios in antagonism assays to identify the antibacterial potential of each strain.
- Explore different culture conditions in at least one probiotic vibrio in order to favor the production of metabolites of interest.
- Obtain extracts and fractions derived from at least one probiotic through chemical extraction and liquid chromatography techniques to be evaluated in in vitro assays.
- Identify the compounds present in the bioactive fractions of at least one probiotic using ultra-efficiency liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS).
- To evaluate the antimicrobial activity of extracts and compounds of at least one vibrio probiotic to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC).
- Analyze the effect on the expression of genes related to growth and pathogenicity mechanisms in V. parahaemolyticus using three concentrations of compounds derived from probiotics.
- To analyze the antimicrobial effect of three concentrations of compounds derived from at least one probiotic vibrio on the morphology of V. parahaemolyticus using microscopy observations.
Participating Institutions:
IKIAM, ESPL, YACHAY.
Participants:
project director Gabriela Salazar Mogollón
- Nina Quilla Espinosa de los Monteros Silva
- Melanie Ashley Ochoa Ocampo
- Jenny Antonia Rodríguez León
- Ricardo Sebastian Palma Patiño
- Edgar Alejandro Pinos Tamayo
- Fabián Orlando Aguilar Mora
- Paul Orlando Guillén Mena
- Jefferson Vladimir Pastuña Fasso
- Cristrian Daniel Quiroz Moreno
Awarded budget: $33000,00
Project status: In process
